Cloning and expression of laccase gene produced from Bacillus subtilis Zhr in E. coli
Other Title(s)
كلونة و تعبير جين اللالكيز Laccase المنتج من بكتريا Bacillus subtilis ZHR في بكتريا E. coli
Joint Authors
Jabbar, Rabah Najah
Hasan, Zaynah Adib
Jabr, Hamid Abbud
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 49, Issue 5 (31 Oct. 2018), pp.879-886, 8 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2018-10-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
Abstract EN
Ten isolates of Laccase producing bacteria were obtained from 50 isolates belongs to Bacillus sp.
These isolates were isolate from different local areas of soil in baghdad.The isolates were subjected to primary screening , and revealed high production of enzyme based on consumed time during the reaction with syringaldazine ( SGZ ) reagent at 37 ° C.
These isolates were subjected to secondary screening.
The results revealed that isolate 136 was the best producers with highest enzyme activity of 47.0 U/ml.
Identification of this isolate was achieved by studying morphological, microscopic characteristic , Vitek2 compact system and studying gene sequencing analysis of 16S rRNA gene .The results revealed that the isolate was belongs to Bacillus subtilis and identified as Bacillus subtilis ZHR.
The laccase gene from Bacillus subtilis ZHR was extracted and amplified by PCR technique, then cloned in expressed Escherichia coli to produce biologically active enzyme with enzyme activity of 108.6 U/ml and specific activity 332.14 U/mg .
American Psychological Association (APA)
Hasan, Zaynah Adib& Jabr, Hamid Abbud& Jabbar, Rabah Najah. 2018. Cloning and expression of laccase gene produced from Bacillus subtilis Zhr in E. coli. The Iraqi Journal of Agricultural Science،Vol. 49, no. 5, pp.879-886.
https://search.emarefa.net/detail/BIM-898077
Modern Language Association (MLA)
Hasan, Zaynah Adib…[et al.]. Cloning and expression of laccase gene produced from Bacillus subtilis Zhr in E. coli. The Iraqi Journal of Agricultural Science Vol. 49, no. 5 (2018), pp.879-886.
https://search.emarefa.net/detail/BIM-898077
American Medical Association (AMA)
Hasan, Zaynah Adib& Jabr, Hamid Abbud& Jabbar, Rabah Najah. Cloning and expression of laccase gene produced from Bacillus subtilis Zhr in E. coli. The Iraqi Journal of Agricultural Science. 2018. Vol. 49, no. 5, pp.879-886.
https://search.emarefa.net/detail/BIM-898077
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 885-886
Record ID
BIM-898077