Evaluation of propolis as non specific immuno-stimulant in fish vaccinated with aeromonas hydrophila vaccine

Abstract EN

This work was designed to study the effect of propolis as immunestimulant on the immune response of fish with or without vaccination using A.hydrophila vaccine.

A total number of 720 apparently healthy catfish {Clarias lazera} were divided into 8 equal groups.

Each group (90 fish) Group 1 served as control.

Fish of groups 2,3 and 4 were injected with proplis (10 %, 20 %, 30 %), respectively.

Fish of group 5,6 and 7 were injected with propolis for a period of 10 days and were vaccinated with formalized- killed vaccine.

Fish of group 8 were vaccinated only with formalized- killed vaccine.

Blood samples were collected periodically to evaluate the indirect heamagglutinating antibodies.

Moreover, all groups were challenged with 0.2 ml of Aeromonas hydrophila.

The relative level of protection (RLP) among the challenged fish was determined.

The antibody titer of indirect hemagglutination test at the 7th day of experiment was 1 in group 1 and 2 in groups 2, 3, 4.

While the groups 5, 8 was 3 but groups 6, 7 was 4.

The antibody titer at the 35th day of the experiment was 1 in group 1 and 4 in groups 2, 3, 4 while the other groups (5, 6, 7), the titer was 10 but group 8 was 3.

The titer at the 42th day of the experiment was 1 in group 1, groups 2, 3, 4 was 2 and in groups 5, 8 was 3 while the groups 6, 7 was 4.

The relative level of protection among the challenged fish of all groups (2-8) were 33.34, 44.45, 55, 56, 88.89, 88.89, 94.45 and 83.84 respectively.

Polyacrylamid gel electrophorectic analysis of serum proteins revealed that propolis increase serum pre-albumin, albumin and globulins.