Detection of CTX-M-type ESBLs from escherichia coli clinical isolates from a tertiary hospital, Malaysia

Abstract EN

The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns.

One hundred of E.

coli isolates were collected from different clinical samples from a tertiary hospital.

ESBL positivity was determined by the disk diffusion method.

PCR used for amplification of CTX-M-type ESBL produced by E.

coli.

Out of 100 E.

coli isolates, twenty-four isolates (24%) were ESBL-producers.

E.

coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%).

After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes.

The CTX-M type ESBL producing E.

coli against antibiotics belonging to different families showed the highest resistance rates to Ampicillin (100%), Cefotaxime (97%), Cefuroxime (95%), and Ciprofoxacin (86%).

Carbapenem groups of antibiotics, Meropenem (89%) and Imipenem (85%) have the highest susceptibility rate among all antibiotics used in this study.

The outcome of the antimicrobial susceptibility testing of significant CTX-M- type ESBL producing E.

coli could be useful to avoid failure or prolong treatments