Detection of escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in al-Qadisiyah province
Other Title(s)
تشخيص مورثة التحلل الدموي للعصيات القولونية و مورثة التسمم المعوي للعنقوديات الذهبية من حليب الجاموس الخام بواسطة تقنية تفاعل السلسلة المتعدد في الوقت الحقيقي في محافظة القادسية
Joint Authors
al-Ubaydi, Janan Nazim Sadiq
Hasan, Hayfa Jumah
Source
Iraqi Journal of Veterinary Sciences
Issue
Vol. 32, Issue 1 (30 Jun. 2018), pp.87-91, 5 p.
Publisher
University of Mosul College of Veterinary Medicine
Publication Date
2018-06-30
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
Abstract EN
The aim of this study was to determines the prevalence of virulence gene hemolysin A (hly A) Escherichia coli and staphylococcal enterotoxins (sea) in Staphylococcus aureus, in raw milk buffaloes.
In molecular laboratory, real-time polymerase chain reaction (RT-PCR) technique has been performed for 24 samples which have been taken randomly from Buffaloes milk, using primers of high specificity for Escherichia coli hlyA gene and Staphylococcus aureus Sea genes.
The results showed different degrees of the studied genes activities.
Four out of 24 samples represented S.
aureus Sea gene (16.6%) whereas 16 out of 24 samples represented E.
coli hlyA gene (66.6%).
this study concluded that buffaloes milk might be a source of contamination with pathogenic bacteria of virulent genes which may have different levels of activities
American Psychological Association (APA)
al-Ubaydi, Janan Nazim Sadiq& Fahd, Khulud Hamdan& Hasan, Hayfa Jumah. 2018. Detection of escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in al-Qadisiyah province. Iraqi Journal of Veterinary Sciences،Vol. 32, no. 1, pp.87-91.
https://search.emarefa.net/detail/BIM-900694
Modern Language Association (MLA)
Fahd, Khulud Hamdan…[et al.]. Detection of escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in al-Qadisiyah province. Iraqi Journal of Veterinary Sciences Vol. 32, no. 1 (2018), pp.87-91.
https://search.emarefa.net/detail/BIM-900694
American Medical Association (AMA)
al-Ubaydi, Janan Nazim Sadiq& Fahd, Khulud Hamdan& Hasan, Hayfa Jumah. Detection of escherichia coli hlyA gene and Staphylococcus aureus Sea gene in raw milk of buffaloes using RT-PCR technique in al-Qadisiyah province. Iraqi Journal of Veterinary Sciences. 2018. Vol. 32, no. 1, pp.87-91.
https://search.emarefa.net/detail/BIM-900694
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 91
Record ID
BIM-900694