Isolation and molecular detection of some virulence genes (eaeA, bfpA) of Escherichia coli from Meat, Milk and cheese
Other Title(s)
دراسة حول العزل و التشخيص الجزيئي لجينات الضراوة للايشريشيا القولونية المعزولة من اللحم، الحليب، و الجبن
Joint Authors
Nasir, Raji Tumah
Mutashshar, Athmar Zahir
Source
University of Thi-Qar Journal of Agricultural Research
Issue
Vol. 7, Issue 2 (31 Dec. 2018), pp.49-62, 14 p.
Publisher
University of Thi-Qar College of Agricultural and Marshes
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
14
Main Subjects
Topics
Abstract EN
Aim: The study was undertaken to isolate diarrheagenic E.
coli from meat and various milk products, and detection of some virulence genes using Polymerase chain reaction (PCR) .
Materials and Methods: Altogether 300 meat and various milk products samples consisting of raw meat (100), cheese (100), milk (100), were collected from milk vendors, retail shops located in Thi-Qar, under aseptic precautions.
For the enrichment of the organism from the collected samples, MacConkey broth was used and inoculation was carried out on MacConkey agar and EMB agar.
Later, to confirm the isolates, various biochemical tests such as IMVC tests, Urease test were performed.
Finally the E.
coli isolates were screened for the presence of virulence associated genes by PCR .
Results: The prevalence of E.
coli was observed in 18.33 % of the samples comprising of meat (25%), cheese (13%), milk (17%).
In this study, out of 55 E.
coli isolates, 18 isolates (32.72%) were positive for eaeA gene, of which 6 (33.3%) isolates from meat, while 5 (27.7%) isolates from milk and 7 (38.88%) isolates from cheese,while 8 isolates (14.5%) were positive for bfpA gene: of which 2(25%) were from each of meat, and 2(25%) for milk, and 4(50%) from cheese.
American Psychological Association (APA)
Mutashshar, Athmar Zahir& Nasir, Raji Tumah. 2018. Isolation and molecular detection of some virulence genes (eaeA, bfpA) of Escherichia coli from Meat, Milk and cheese. University of Thi-Qar Journal of Agricultural Research،Vol. 7, no. 2, pp.49-62.
https://search.emarefa.net/detail/BIM-901912
Modern Language Association (MLA)
Mutashshar, Athmar Zahir& Nasir, Raji Tumah. Isolation and molecular detection of some virulence genes (eaeA, bfpA) of Escherichia coli from Meat, Milk and cheese. University of Thi-Qar Journal of Agricultural Research Vol. 7, no. 2 (2018), pp.49-62.
https://search.emarefa.net/detail/BIM-901912
American Medical Association (AMA)
Mutashshar, Athmar Zahir& Nasir, Raji Tumah. Isolation and molecular detection of some virulence genes (eaeA, bfpA) of Escherichia coli from Meat, Milk and cheese. University of Thi-Qar Journal of Agricultural Research. 2018. Vol. 7, no. 2, pp.49-62.
https://search.emarefa.net/detail/BIM-901912
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 57-60
Record ID
BIM-901912