Development of antibiotic detection bioassay kit from locally isolated bacteria
Other Title(s)
تطوير فحص حيوي للكشف عن متبقيات المضادات الحيوية باستخدام بكتيريا معزولة محليا
Dissertant
Thesis advisor
al-Nabris, Kamal Jad Allah
al-Manamah, Abd al-Rauf Ali
University
Islamic University
Faculty
Faculty of Science
Department
Department of Biological Science
University Country
Palestine (Gaza Strip)
Degree
Master
Degree Date
2017
English Abstract
Background: The presence of antibiotic residues in milk may cause different diseases or disorders like, direct toxic effects, allergic reactions in individuals with hypersensitivity, and can result in the development of resistant strains of bacteria in consumers.
In addition, these residues can modify or inhibit the fermentation processes performed in dairy products such as cheese and yoghurt.
The determination of antibiotic residues in raw milk is usually performed by two methods.
Microbial and enzymatic methods, like microbial growth inhibition tests.
Tests utilize bacterial test strains such as Bacillus stearothermophilus var.
calidolactis, Streptococcus thermophilus and Bacillus subtilis ATCC 6633.
Objective: The main objective of this study is to develop antibiotic residues detection bioassay kit from locally isolated bacteria from soil sample in Gaza strip.
Methods: soil samples were collected from different locations in the three governorates at Gaza strip.116 bacteria isolates were isolated from soil sample.
The bacteria isolates were identified as the most resistance of all antibiotics.
They allowed to grow in different cultures at 60 ° C.
After that, a suitable the culture media developed for growing of the isolates bacteria.
Collecting 81 milk samples and then determination the containing antibiotic residues using a commercial test (Mira test) and the locally developed test .
Results: .
The percentage of presumably positive results after 4 hours experiment was 82.7, 79.0 and 76.5 for the LDMBB-4 h, LDMBG-4 h and MiRA Test-4 h respectively.
After 24 h, this percentages were dropped to 49.4, 35.8 and 17.3% in LDMBB-24 h, LDMBG-24 h, and MiRA Test-24h respectively.
Results of the chi-square test of homogeneity revealed a statistically significant difference (p < 0.001) in proportions of positive (or negative) residues in milk samples between the six experimental trials.
Conclusions: The result was statistically significant compared to locally kit and commercial kit.
Therefore, we recommend that used for locally kit, when to use it positive effects on consumer health
Main Subjects
No. of Pages
64
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Results and discussion.
Chapter Five : Conclusions and recommendations.
References.
American Psychological Association (APA)
al-Kurd, Ayat Ahmad Shukri. (2017). Development of antibiotic detection bioassay kit from locally isolated bacteria. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-902790
Modern Language Association (MLA)
al-Kurd, Ayat Ahmad Shukri. Development of antibiotic detection bioassay kit from locally isolated bacteria. (Master's theses Theses and Dissertations Master). Islamic University. (2017).
https://search.emarefa.net/detail/BIM-902790
American Medical Association (AMA)
al-Kurd, Ayat Ahmad Shukri. (2017). Development of antibiotic detection bioassay kit from locally isolated bacteria. (Master's theses Theses and Dissertations Master). Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-902790
Language
English
Data Type
Arab Theses
Record ID
BIM-902790
