Molecular detection of Pseudomonas aeruginosa isolated from minced meat and studies the pyocyanin effectiveness on pathogenic bacteria
Other Title(s)
الكشف الجزيئي لبكتريا Pseudomonas aeruginosa المعزولة من اللحم المثروم و دراسة الفعالية البيولوجية لمادة البايوسيانين ضد البكتريا المرضية
Author
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 50, Issue 4 (31 Aug. 2019), pp.1199-1207, 9 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2019-08-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
This study was aimed to collected Minced meat from the local markets in Baghdad governorate during 2018, and examined for the presence of Pseudomonas aeruginosa, in order to extract and purify pyocyanin and examined it as an antimicrobial activity against pathogenic bacteria in foods.
Fifteen isolates were isolated from 50 samples and identified as P.
aeruginosa using the API20E system and finally confirmed with PCR using 16SrRNA gene.
Four tested media were used for the production of pigment after incubation within 72 h, One strain which given a vigorous pigmentation was chosen and extracted with chloroform and HCl then analyzed with Gas chromatography (GC-Mass) which showed a sharp peak at the time of acquisition of 27.13 minutes at the chromatographic analysis recognized with mass spectrometry as Hemipyocanin (alpha-hydroxy phenazine) which produced molecular ion with intensive peak at 205 m/z.
Agar well diffusion technique was applied for estimating the antimicrobial activity of purified (pyocyanin) with variable concentrations (25, 50, 75 and 100 mg/ml) which monitored toward Gram-negative and Gram-positive bacteria that isolated of minced meat.
Escherichia coli and staphylococcus aureus was the most affected with pyocyanin were followed by Serratia marcescens and Klebsiella sp.
at the same level.
While Enterobacter sp, Bacillus cereus, Proteus mirabilis, and Proteus vulgaris showed intermediate sensitivity, the Pseudomonas fluorescens was shown low sensitivity to pyocyanin.
American Psychological Association (APA)
Qasim, Dua Adil. 2019. Molecular detection of Pseudomonas aeruginosa isolated from minced meat and studies the pyocyanin effectiveness on pathogenic bacteria. The Iraqi Journal of Agricultural Science،Vol. 50, no. 4, pp.1199-1207.
https://search.emarefa.net/detail/BIM-903885
Modern Language Association (MLA)
Qasim, Dua Adil. Molecular detection of Pseudomonas aeruginosa isolated from minced meat and studies the pyocyanin effectiveness on pathogenic bacteria. The Iraqi Journal of Agricultural Science Vol. 50, no. 4 (2019), pp.1199-1207.
https://search.emarefa.net/detail/BIM-903885
American Medical Association (AMA)
Qasim, Dua Adil. Molecular detection of Pseudomonas aeruginosa isolated from minced meat and studies the pyocyanin effectiveness on pathogenic bacteria. The Iraqi Journal of Agricultural Science. 2019. Vol. 50, no. 4, pp.1199-1207.
https://search.emarefa.net/detail/BIM-903885
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-903885