Chronic sleepdeprivation effects on ischemia-reperfusion injury : role of the sympathetic nervous syste

Abstract EN

Objective: Chronic sleep deprivation has disturbing effects on health.The aim of this study is to assess the effects of chronic sleep deprivation on myocardial ischemiareperfusion injury.

Methods:Male Wistar rats were randomly divided into four groups;the heart of all the animals was perfused in the Langendorff apparatus using Krebs-Henseleit buffer.

Hearts were allowed a 15-minute recovery which was considered as baseline, then underwent 30 minutes ischemia followed by 60 minutes reperfusion.

Grouping: 1) Ischemia/ reperfusion group (IR); 2) Control group (CON): rats were placed in large multiple platforms for 72 hours prior to IR; 3) Chronic sleep deprivation group (CSD): 72 hours sleep deprivation was induced using small multiple platforms prior to IR;4) Sympathectomy group (SYMP):chemical sympathectomy was done 24 hours before chronic sleep deprivation induction followed by subjection to IR.

Outcome measures included heart rate (HR), left ventricular developed pressure (LVDP), rate product pressure (RPP) and the ratio of infarct size to area at risk (IS/AAR %).

Results:Our results showed IS/AAR considerably increased in the IR group as compared to the sleep deprivation group ( p < 0.001); and sympathectomy could decrease infarct size as compared to sleep deprivation ( p < 0.001).

There were no significant differences between HR, LVDP and RPP between groups.

Conclusions: Induction of 72 h sleep deprivation following ischemia and reperfusion increased the infarct size in a sympathetic dependent manner, and this effect of sleep deprivation is not related to changes in hemodynamic parameters.