Evaluation of pap and sfa genes relative frequency p and s fimbriae encoding of uropathogenic escherichia coli isolated from hospitals and medical laboratories, Yasuj city, Southwest Iran
Joint Authors
Khuramruz, Sayyid Sajad
Sharifi, Asghar
Khosravani, Sayyid Abd al-Majid
Borumand, Mustafa
Munzouri, Layla
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 21, Issue 8 (31 Aug. 2019), pp.1-8, 8 p.
Publisher
Publication Date
2019-08-31
Country of Publication
United Arab Emirates
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background : urinary tract infections (UTI) are one of the common infections worldwide.
escherichia coli is the most common bacteria causing UTI.
Objectives : the current study aimed at investigating the susceptibility pattern, biofilm formation, and the relationship between pap and SFA virulence genes and biofilm formation in e.
coli strains isolated from patients with UTI from 2017 to 2018 in yasuj city, Iran.
Methods : in the current cross-sectional study, 130 e.
coli strains were isolated from patients with UTI.
antimicrobial susceptibility testing was performed by disk diffusion method.
the presence of pap and SFA genes was investigated by multiplex polymerase chain reaction (PCR).
biofilm formation was tested using microtiter plate assay and Elisa (enzyme-linked immunosorbent assay) reader for e.
coli isolates.
results : out of 130 e.
coli strains isolated from UTI samples, 98 (75.38%) were positive for the biofilm formation, (14% strong, 31% moderate, and 53% weak).
the pap and SFA genes were detected in 50% and 29% of the isolates, respectively.
majority of the isolates were resistant against ampicillin (80%) and susceptible to amikacin and meropenem (100%); the pap and sfa genes were detected in 92.85% and 85.71% of the biofilm-forming isolates, respectively.
these genes in uropathogenic e.
coli (upec) have a significant relationship with strong biofilm formation (p < 0.0001).
conclusions : the obtained results indicated that the frequency of pap gene was higher than that of SFA in e.
coli strains isolated from patients with UTI in yasuj.
the pap and SFA genes in e.
coli strains had a significant relationship with strong biofilm formation.
in addition, the frequency of biofilm formation in antibiotic-resistant e.
coli was higher than that of antibiotic-sensitive strains.
according to the results of the current study, it is recommended to administer amikacin or meropenem in yasuj to treat UTI.
American Psychological Association (APA)
Borumand, Mustafa& Sharifi, Asghar& Munzouri, Layla& Khuramruz, Sayyid Sajad& Khosravani, Sayyid Abd al-Majid. 2019. Evaluation of pap and sfa genes relative frequency p and s fimbriae encoding of uropathogenic escherichia coli isolated from hospitals and medical laboratories, Yasuj city, Southwest Iran. Iranian Red Crescent Medical Journal،Vol. 21, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-904308
Modern Language Association (MLA)
Borumand, Mustafa…[et al.]. Evaluation of pap and sfa genes relative frequency p and s fimbriae encoding of uropathogenic escherichia coli isolated from hospitals and medical laboratories, Yasuj city, Southwest Iran. Iranian Red Crescent Medical Journal Vol. 21, no. 8 (Aug. 2019), pp.1-8.
https://search.emarefa.net/detail/BIM-904308
American Medical Association (AMA)
Borumand, Mustafa& Sharifi, Asghar& Munzouri, Layla& Khuramruz, Sayyid Sajad& Khosravani, Sayyid Abd al-Majid. Evaluation of pap and sfa genes relative frequency p and s fimbriae encoding of uropathogenic escherichia coli isolated from hospitals and medical laboratories, Yasuj city, Southwest Iran. Iranian Red Crescent Medical Journal. 2019. Vol. 21, no. 8, pp.1-8.
https://search.emarefa.net/detail/BIM-904308
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-904308