Protective role of the combination of resveratrol and N-acetylcysteine in preventing acute paracetamol-induced nephropathy in rats
Other Title(s)
الدور الوقائي لمزيج من الريسفيراترول و ن-أسيتيل سيستين لمنع اعتلال الكلى الحاد الناتج عن الباراسيتامول في الجرذان
Author
Source
Scientific Journal of King Faisal University : Basic and Applied Sciences
Issue
Vol. 19, Issue 1 (30 Jun. 2018), pp.39-55, 17 p.
Publisher
Publication Date
2018-06-30
Country of Publication
Saudi Arabia
No. of Pages
17
Main Subjects
Abstract EN
As an overdose of Paracetamol (PCM) is nephrotoxic, the present study aimed to investigate and compare the individual therapeutic effects of Resveratrol (RES), N-acetyl cysteine (NAC), and their combination against PCM induced nephrotoxicity in rats in addition to illustrate possible mechanisms of action.
Fifty adult Wistar rats were divided into 5 groups (n=10/group): 1) control group, 2) PCM group (1000 mg/kg b.wt), 3) PCM post-treated with RES (25 mg/kg b.wt), 4) PCM post-treated with NAC (1.2 g/kg b.wt), and 5) PCM post-treated with concomitant RES and NAC.
PCM was administered during the first 2 days and all other treatments were administered intraperitoneally daily for the post PCM induction five days.
At the end of the study, serum electrolytes, renal levels of oxidative stress marker, mRNA expression levels of ɣ-glutamyl cysteine ligase (ɣ-GCL), and Na+/K+ ATPase were measured.
PCM intoxication caused oxidative stress-induced renal damage as evident by the inhibition of mRNA expression of ɣ-GCL and glutathione (GSH) levels, and by the inhibition of the activities of superoxide dismutase (SOD), catalase (CAT), and glutathione-S-transferase (GST).
PCM induced renal damage was mediated by a parallel decrease in activity and expression levels of Na+/K+ ATPase.
However, while delayed NAC administration to post PCM intoxicated rat poorly improved these parameters and changes in kidney structure, the effect of RES was also partially but more profound.
On the other hand, concomitant administration of both NAC and RES completely reversed the alterations of serum electrolytes, renal architectures histological parameters, serum electrolytes, oxidative stress parameters, renal architectures histological parameters, and resulted in normal renal mRNA expression of both ɣ-GCL and Na+/K+ ATPase.
These data suggest that NAC and RES act in a synergistic manner and completely ameliorate PCM induced nephrotoxicity.
American Psychological Association (APA)
Shati. Ali A.. 2018. Protective role of the combination of resveratrol and N-acetylcysteine in preventing acute paracetamol-induced nephropathy in rats. Scientific Journal of King Faisal University : Basic and Applied Sciences،Vol. 19, no. 1, pp.39-55.
https://search.emarefa.net/detail/BIM-908329
Modern Language Association (MLA)
Shati. Ali A.. Protective role of the combination of resveratrol and N-acetylcysteine in preventing acute paracetamol-induced nephropathy in rats. Scientific Journal of King Faisal University : Basic and Applied Sciences Vol. 19, no. 1 (Jun. 2018), pp.39-55.
https://search.emarefa.net/detail/BIM-908329
American Medical Association (AMA)
Shati. Ali A.. Protective role of the combination of resveratrol and N-acetylcysteine in preventing acute paracetamol-induced nephropathy in rats. Scientific Journal of King Faisal University : Basic and Applied Sciences. 2018. Vol. 19, no. 1, pp.39-55.
https://search.emarefa.net/detail/BIM-908329
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-908329