Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate
Joint Authors
al-Kabi, Hana Jafar Jabbar
al-Hamdani, Maytham Ayyub
Source
مجلة جامعة الكوفة لعلوم الحياة : مجلة علمية محكمة
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2018-12-31
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Topics
English Abstract
The present study aimed to Purification and characterization of lipase produced by the isolate Pseudomonas aeruginosa.
The results showed that crude lipase was purified by using the precipitation step of several concentrations ratios of cold acetone.
The concentration ratio 4:1 gave the best specific activity 6 U/mg of protein.
The subsequent purification step using gel filtration was showed that specific activity reached to 40.5U/mg of protein.
The gel electrophoresis (SDS-PAGE) of lipase purity were determined.
the result showed there is an appearance of a single protein band predominant in all purification steps, While The molecular weight of purified lipase was 68KDa
Data Type
Conference Papers
Record ID
BIM-910920
American Psychological Association (APA)
al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. 2018-12-31. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. International Scientific Conference (3rd : 2018 : Najaf, Iraq). . Special issue (2018), pp.138-145.Najaf Iraq : University of Kufa Faculty of Science Department of live Sciences.
https://search.emarefa.net/detail/BIM-910920
Modern Language Association (MLA)
al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. . Najaf Iraq : University of Kufa Faculty of Science Department of live Sciences. 2018-12-31.
https://search.emarefa.net/detail/BIM-910920
American Medical Association (AMA)
al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. . International Scientific Conference (3rd : 2018 : Najaf, Iraq).
https://search.emarefa.net/detail/BIM-910920
