A genetic polymorphism of interleukin 10 gene at position-1082 in Iraqis patients with type 1 diabetes of

Abstract EN

The aim of this study was to evaluate the frequency of polymorphism of interleukin-10 Gene at position-1082 of the promoter region (IL-10-1082 SNP) in T1D and in healthy.

The subjects were determined in 39 Iraqi patients, (12 males & 27 females; 1 5.65 ± 1.79 years) and 21 controls (7 male and 14 female; 14.66 ± 3.43 years) by polymerase chain reaction-specific sequence primer (PCR-SSP) assay, followed by electrophoresis on agarose-gel.

The results revealed that comparing IL10 -1082 genotypes and alleles between T1D patients and controls it was observed that frequency of GA genotype was significantly higher (56.41vs.

42.85%; RR =23.7).

may be considered as a risk factor for T1D While A allele was not significantly in patients contrast to controls, (41.03vs.

40.48%; RR =0.9) and the associated EF values were 1.73and 1.02, respectively.

In contrast, GG genotype and G allele (30.76vs.

58.97%, P =0.579 respectively) frequencies were significantly decreased in patients, compared to controls (38.09vs.

59.52%; P =1.000), and the associated PF values were 0.72 and 0.98, respectively .

Similar observations were made in AA genotype.

These findings suggest that IL10-1082 polymorphism showed associations (positive and negative) with T1D in the samples of Iraqi patients .

Therefore, the functional role of such IL10-1082 might have been altered due to the deviations of some genotype and allele frequencies.