Antioxidant activity of water extract of propolis from different regions in kafr el-sheikh governorate

Abstract EN

Water extracts of propolis collected from three geographic regions (Motobes, Kafr El-Sheikh and Desouk) in Kafr El-Sheikh Governorate, Egypt were prepared.

The extracts were analyzed for the determination of total polyphe-nols which ranged from 5.70 to 8.79 g / 100 g of the sample and from 22.80 to 34.30 g / 100 g of the freeze-dried extract.

the total flavonoid content ranged from 3.05 to 4.85 g / 100 g of the sample.

Water extracts of propolis were evaluated for antioxidant activities using the β- carotene bleaching and 1,1-diphenyl-2- picrylhydrazyl (DPPH) free radical-scavenging assay systems.

It was observed that all propolis had strong antioxidant activities due to their contents of total polyphenol and flavonoid.

The highest activities were found for samples from Desouk followed by these from Kafr El-Sheikh, then those from Motobes.

Freeze-dried extracts of propolis can be used as natural antioxidants in sunflower oil as compared to BHT and TBHQ.

Propolis from Desouk and Kafr El-Sheikh at 200 and 300 ppm were similar in reducing peroxide values and both of them at 300 ppm were better than BHT but lower than TBHQ, added at 200 ppm concentration, in reducing peroxides and hydroperoxides production in sunflower oil at 63 °C for 4 days.