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Use of immobilized L-arabinose isomerase for prodduction of tagatose
Other Title(s)
استعمال أنزيم L-arabinose isomerase المقيد في إنتاج سكر التاكاتوز Tagatose
Author
al-Sufi, Muhammad Abd al-Razzaq
Source
Iraq Journal of Market Research and Consumer Protection
Issue
Vol. 11, Issue 2 (31 Dec. 2019), pp.122-131, 10 p.
Publisher
University of Bagdad Market Research and Consumer Protection Center
Publication Date
2019-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
L-arabinose isomerase from Escherichia coli O157:H7 Was immobilized with activated Bentonite from local markets of Baghdad, Iraq by 10% 3-APTES and treated with 10% aqueous glutaraldehyde, the results refer that the yield of immobilization was 89%, and pH profile of free and immobilized L-arabinose isomerase was 7 and 7.5 and it is stable at 6-8 for 60 min respectively, while, the optimum temperature was 30 and 35°C and it was stable at 35 and 40°C for 60 min but it loses more than 60 and 30% from its original activity at 50°C for free and immobilized L-arabinose isomerase respectively.
Immobilized enzyme retained its full activity for 32 day, but it retained 73.58% of its original activity after storage for 60 day at 4°C, and its retained a full activity for 36 continue usage; while it retained 84.63% of its original activity after 50 continue usage.
Immobilized enzyme could to get about 85% of D-tagatose from 100 gmL of D-galactose as a substrate with at 80 rpm of reaction speed for 24 hr.
American Psychological Association (APA)
al-Sufi, Muhammad Abd al-Razzaq. 2019. Use of immobilized L-arabinose isomerase for prodduction of tagatose. Iraq Journal of Market Research and Consumer Protection،Vol. 11, no. 2, pp.122-131.
https://search.emarefa.net/detail/BIM-945686
Modern Language Association (MLA)
al-Sufi, Muhammad Abd al-Razzaq. Use of immobilized L-arabinose isomerase for prodduction of tagatose. Iraq Journal of Market Research and Consumer Protection Vol. 11, no. 2 (2019), pp.122-131.
https://search.emarefa.net/detail/BIM-945686
American Medical Association (AMA)
al-Sufi, Muhammad Abd al-Razzaq. Use of immobilized L-arabinose isomerase for prodduction of tagatose. Iraq Journal of Market Research and Consumer Protection. 2019. Vol. 11, no. 2, pp.122-131.
https://search.emarefa.net/detail/BIM-945686
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 130-131
Record ID
BIM-945686