Molecular detection of astrovirus using reverse transcriptase quantitative polymerase chain reaction technique
Other Title(s)
التحري الجزيئي للفايروس النجمي باستخدام تقنية تفاعل سلسلة البلمرة عكس الاستنساح اللحظي
Joint Authors
al-Hamdani, Faysal Ghazi
Fadil, Hala Yunus
Abd al-Karim, Mais Jasim
Awfi, Iman Mutashshar
Source
The Iraqi Journal of Agricultural Science
Issue
Vol. 51, Issue (s) (31 Jan. 2020), pp.191-200, 10 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2020-01-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the effectiveness of the AstV infection on the vaccine immunogenicity in the vaccinated infected children.
By RT-qPCR based Sybre green associatedmelting curve assay, stool samples of 49 enterovirus suspected patients and of 39 rotavirus suspected patients were tested for AstV.
Results of EVs group showed a 29 (59.2%) positive AstV contributed to 26 (89.5%) co-infection with Evs and 3 (10.3%) as a single infection in negative samples for Evs.
Furthermore, AstV co-infection percentage is higher than the single infection.
Moreover, the percentage of the Astrovirus among the vaccinated AFP-suspected cases was 53%, while the percentage of these viruses among the unvaccinated was 100%.
Thus, MamAstrovirus- 1 MK948878 is the first local isolate recorded in the Genbank.
In conclusion, the RT-qPCR based on SYBR Green showed the rapid and efficient detection of AstV with few copies number.
This allow to be used for the diagnosis of AstV along with other gastroenteritis viruses in a multiplex assay to reduce processing time.
American Psychological Association (APA)
Fadil, Hala Yunus& Abd al-Karim, Mais Jasim& Awfi, Iman Mutashshar& al-Hamdani, Faysal Ghazi. 2020. Molecular detection of astrovirus using reverse transcriptase quantitative polymerase chain reaction technique. The Iraqi Journal of Agricultural Science،Vol. 51, no. (s), pp.191-200.
https://search.emarefa.net/detail/BIM-948729
Modern Language Association (MLA)
Fadil, Hala Yunus…[et al.]. Molecular detection of astrovirus using reverse transcriptase quantitative polymerase chain reaction technique. The Iraqi Journal of Agricultural Science Vol. 51, Special Issue (2020), pp.191-200.
https://search.emarefa.net/detail/BIM-948729
American Medical Association (AMA)
Fadil, Hala Yunus& Abd al-Karim, Mais Jasim& Awfi, Iman Mutashshar& al-Hamdani, Faysal Ghazi. Molecular detection of astrovirus using reverse transcriptase quantitative polymerase chain reaction technique. The Iraqi Journal of Agricultural Science. 2020. Vol. 51, no. (s), pp.191-200.
https://search.emarefa.net/detail/BIM-948729
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 198-200
Record ID
BIM-948729