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Evaluation of breeding programs susceptibility for two important forage crops using DNA Barcoding
Other Title(s)
تقييم قابلية نوعين من محاصيل العلف لبرامج التربية باستخدام تقنية DNA Barcoding
Author
Source
Alexandria Science Exchange Journal
Issue
Vol. 38, Issue 1 (31 Mar. 2017), pp.27-37, 11 p.
Publisher
Publication Date
2017-03-31
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
This investigation was carried out to identify and evaluate Medicago sativa andTrifolium alexandrinum probability for breeding program based on two bar-coding genes (rbcl and Cox1 genes).
Identification of Medicago sativa Baladi 1 was performed through rbcl and Cox1 genes.
Medicago sativa Baladi 1 was identified as Medicago sativa voucher G00199095 ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene, partial cds; chloroplast (Sequence ID: KJ204375.1) and Medicago sativa voucher Ahrendsen_23 for rbcl and Cox1 genes respectively.
Identity values were recorded with 90% of identity for alfalfa, Baladi 1 Genotype ribulose – 1/5 – bisphosphate carboxylase / oxygenase large subunit (rbcl) gene (sequences ID: KJ206375.1) also, identity values were recorded with 91.24% of identity for alfalfa Baladi 1 Genotype, cytochrome c oxiddase bubunit I gene (cox 1) (sequence ID: KJ 204375.1).
Trifolium alexandrinum Helaly genotype was identified as Trifolium alexandrinum (Sequence ID: HM850407.1) and Trifolium alexandrinum voucher K-016Hv (Sequence ID: KU234213.1) as rbcl and Cox1 genes respectively.
Affiliation of genetic origin was detected for Trifolium alexandrinum with 100 % of similarity with origin source which indicate highly possibility for applying breeding programs comparing with Medicago sativa which reflect the lowest genetic similarity with origin source.
American Psychological Association (APA)
Bunduq, Abd al-Aziz Talat. 2017. Evaluation of breeding programs susceptibility for two important forage crops using DNA Barcoding. Alexandria Science Exchange Journal،Vol. 38, no. 1, pp.27-37.
https://search.emarefa.net/detail/BIM-952478
Modern Language Association (MLA)
Bunduq, Abd al-Aziz Talat. Evaluation of breeding programs susceptibility for two important forage crops using DNA Barcoding. Alexandria Science Exchange Journal Vol. 38, no. 1 (Jan. / Mar. 2017), pp.27-37.
https://search.emarefa.net/detail/BIM-952478
American Medical Association (AMA)
Bunduq, Abd al-Aziz Talat. Evaluation of breeding programs susceptibility for two important forage crops using DNA Barcoding. Alexandria Science Exchange Journal. 2017. Vol. 38, no. 1, pp.27-37.
https://search.emarefa.net/detail/BIM-952478
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-952478