Molecular characterization of carbapenem resistance in clinical isolates of Acinetobacter baumannii and therapeutic options

Dissertant

Hamdan, Murad Sulayman

Thesis advisor

Farraj, Muhammad

University

Birzeit University

Faculty

Faculty of Pharmacy, Nursing and Health Professions

University Country

Palestine (West Bank)

Degree

Master

Degree Date

2019

English Abstract

Acinetobacter baumannii is gram negative coccobacilli characterized by being non-motile, oxidase negative.

It has been recognized to colonize hospitalized patients particularly the elderly, infants and the immunocompromised thus causing severe infections with high morbidity and mortality.

A.

baumannii plays a predominant role in causing nosocomial pneumonia particularly the ventilation-related pneumonia seen among patients in intensive care units.

A.

baumannii has been classified as one of the six most important multidrug-resistant microorganisms in hospitals worldwide.

Although its resistance to all known antibiotics has appeared, most strains remain susceptible to carbapenems.

Therefore, treatment options are limited to carbapenems, colistin and Tigecycline.

The aim of this study was to screen for carbapenemase producing A.

baumannii clinical isolates.

The study has been conducted on 59 hospitalized patients and outpatients attending clinics throughout the West Bank, Palestine.

The samples were collected between October 2016 and November 2017.

Acinetobacter baumannii clinical isolates were obtained from different sources including urine, wound, blood, sputum and tracheal aspirates.

Antibiotic susceptibility testing by the disk diffusion method and minimal inhibitory concentration by the microbroth dilution method was performed following the guidelines of the Clinical and Laboratory Standards Institute.

Polymerase gene reaction was performed to determine the genes involved in carbapenem resistance.

The genes tested include the OXA-b-lactamases; OXA 51, OXA 23, OXA 24 and OXA 58.

The results obtained indicated that all isolates were resistant to Meropenem.

Susceptibility to colistin and Tigecycline were 84.7% (50/59) and 88.1% (52/59) respectively PCR results showed that all blaOXA51 gene was carried by all isolates while blaOXA523 gene was carried by 54.2% (32/59) of isolates.

None of the Acinetobacter isolates tested carried the blaOXA24 and blaOXA58 genes.

blaOXA51 gene has been found to be carried by all strains of A.

baumannii and usually used to confirm the identification of the clinical isolates.

Regarding the blaOXA24 and blaOXA58 genes, It has been reported that A.

baumannii carrying these two genes show have resistance to carbapenems.

However, these two genes are are not universally spread but reported in certain areas in the world such as Spain and France.

Regarding blaOXA-23, our finding is consistent with published literature regarding the spread of carbapenem resistance in A.

baumannii isolates carrying this gene.

Main Subjects

Nursing

Topics

No. of Pages

43

Table of Contents

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction and literature review.

Chapter Two : Aims of the study.

Chapter Three : Materials and methods.

Chapter Four : Results.

Chapter Five : Discussion and recommendations

References.

American Psychological Association (APA)

Hamdan, Murad Sulayman. (2019). Molecular characterization of carbapenem resistance in clinical isolates of Acinetobacter baumannii and therapeutic options. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-957350

Modern Language Association (MLA)

Hamdan, Murad Sulayman. Molecular characterization of carbapenem resistance in clinical isolates of Acinetobacter baumannii and therapeutic options. (Master's theses Theses and Dissertations Master). Birzeit University. (2019).
https://search.emarefa.net/detail/BIM-957350

American Medical Association (AMA)

Hamdan, Murad Sulayman. (2019). Molecular characterization of carbapenem resistance in clinical isolates of Acinetobacter baumannii and therapeutic options. (Master's theses Theses and Dissertations Master). Birzeit University, Palestine (West Bank)
https://search.emarefa.net/detail/BIM-957350

Language

English

Data Type

Arab Theses

Record ID

BIM-957350