Natural Killer T cells in Egyptian patients with HCV-associated Non-alcoholic fatty liver disease
Joint Authors
Hasubah, Huwayda M.
Ali, Raghdah K.
Hasan, Adil A.
al-Saqqa, Amr A.
Imad, Rasha
Yunus, Suha Izz al-Din
Hasan, Amani M.
Source
Suez Canal University Medical Journal
Issue
Vol. 22, Issue 1 (30 Jun. 2019), pp.95-105, 11 p.
Publisher
Suez Canal University Faculty of Medicine
Publication Date
2019-06-30
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Topics
Abstract EN
Background:Studies on Natural Killer T (NKT) cells in Non-alcoholic fatty liver disease (NAFLD) have shown contradictory results.
Moreover, the effect of HCV superinfection on NKT cells in NAFLD patients have not been previously studied.
Aim: to assess the effect of the concomitant HCV infection on the frequency of NKT cells and CD8+ T cells in a cohort of Egyptian patients with hepatic steatosis.
Subjects and Methods: Two-hundred subjects were divided into four equal groups, 1) concomitant hepatic steatosis/HCV, 2) HCV (without steatosis), 3) steatosis (without HCV), and 4) normal controls.
NKT and CD8+ T cells were identified by monoclonal antibodies surface staining for (CD3, CD56, CD161), and (CD3, CD8) respectively using flow cytometry.
Results: CD3+/CD56+/CD161+ NKT cells were significantly higher in patients with either steatosis (alone) or concomitant HCV/steatosis compared to control (p<0.0001 each), while comparable NKT frequency was found between HCV group and control (p=0.72).
A significant increase in CD3+/CD8+ T cells was noted in the three patients’ groups compared to control (p=0.0001, 0.002 and 0.001, respectively).
Interestingly, NKT cells were significantly higher in patients with steatosis/HCV compared to those with HCV alone (p<0.0001), while, they did not differ betweenconcomitant HCV/steatosis and steatosis (alone) group.
Higher frequencies of both NKT cells and CD8+ T cells were significantly associated with increased severity of steatosis.
In HCV group, NKT frequencies were unrelated to steatosis severity.
Conclusion: NKT cells are significantly elevated in patients with hepatic steatosis irrespective to the presence or absence of concomitant HCV infection.
American Psychological Association (APA)
Ali, Raghdah K.& Yunus, Suha Izz al-Din& Hasan, Adil A.& Hasan, Amani M.& al-Saqqa, Amr A.& Imad, Rasha…[et al.]. 2019. Natural Killer T cells in Egyptian patients with HCV-associated Non-alcoholic fatty liver disease. Suez Canal University Medical Journal،Vol. 22, no. 1, pp.95-105.
https://search.emarefa.net/detail/BIM-958786
Modern Language Association (MLA)
Ali, Raghdah K.…[et al.]. Natural Killer T cells in Egyptian patients with HCV-associated Non-alcoholic fatty liver disease. Suez Canal University Medical Journal Vol. 22, no. 1 (2019), pp.95-105.
https://search.emarefa.net/detail/BIM-958786
American Medical Association (AMA)
Ali, Raghdah K.& Yunus, Suha Izz al-Din& Hasan, Adil A.& Hasan, Amani M.& al-Saqqa, Amr A.& Imad, Rasha…[et al.]. Natural Killer T cells in Egyptian patients with HCV-associated Non-alcoholic fatty liver disease. Suez Canal University Medical Journal. 2019. Vol. 22, no. 1, pp.95-105.
https://search.emarefa.net/detail/BIM-958786
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 103-105
Record ID
BIM-958786