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Production of aspartame by immobilized Thermolysin
Other Title(s)
إنتاج سكر الأسبارتام باستعمال أنزيم Thermolysin المقيد
Joint Authors
Aziz, Raghad Akram
al-Sufi, Muhammad Abd al-Razzaq
Source
Issue
Vol. 60, Issue 6 (30 Jun. 2019), pp.1232-1239, 8 p.
Publisher
University of Baghdad College of Science
Publication Date
2019-06-30
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
The aim of this study was the production of aspartame by using immobilized thermolysin in bentonite clay.
The yield of immobilized thermolysin in bentonite was 92% of the original enzyme amount.
pH profile of free and immobilized enzyme was 7.0 and 7.5 respectively which was stable at 6.5-9.0 for 30min.
The optimum temperature of both enzymes was 50°C, while they were stable at 65°C for 30min.
however, they lost 52.73 and 61.72% from its main activity at 80°C respectively.
Immobilized thermolysin has retained all activity within 27 days, but it kept 68.27% of initial activity when stored for 60 days at 4°C whereas, it retained a full activity after 20 continue usage.
In addition, it retained 86.53% of its original activity after 30 continuing usages.
The yield of produced aspartame was increased with reaction time; it was 9% after 1h and increased gradually to 100% after 10h at reaction conditions.
American Psychological Association (APA)
al-Sufi, Muhammad Abd al-Razzaq& Aziz, Raghad Akram. 2019. Production of aspartame by immobilized Thermolysin. Iraqi Journal of Science،Vol. 60, no. 6, pp.1232-1239.
https://search.emarefa.net/detail/BIM-969353
Modern Language Association (MLA)
al-Sufi, Muhammad Abd al-Razzaq& Aziz, Raghad Akram. Production of aspartame by immobilized Thermolysin. Iraqi Journal of Science Vol. 60, no. 6 (2019), pp.1232-1239.
https://search.emarefa.net/detail/BIM-969353
American Medical Association (AMA)
al-Sufi, Muhammad Abd al-Razzaq& Aziz, Raghad Akram. Production of aspartame by immobilized Thermolysin. Iraqi Journal of Science. 2019. Vol. 60, no. 6, pp.1232-1239.
https://search.emarefa.net/detail/BIM-969353
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-969353