Improve of indulines enzyme productivity from recombinant Escherichia coli BL21 (DE3)
Author
Source
Mesopotamia Environment Journal
Issue
Vol. 5, Issue 4 (30 Jun. 2020), pp.51-57, 7 p.
Publisher
University of Babylon Environmental Research and Studies Center
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Topics
Abstract EN
In the present study, the effects of process parameters on inulinase production by recombinant Escherichia coli BL21 (DE3) have been studied by pH of medium, incubation time, shaking speed of incubation (rpm), hydrolysis of Inulin and effect of metal ion in activity of enzyme.
The results indicated that increased of production inulinaseenzyme by change in parameter of which the optimal incubation time was 8 h, the maximal activity and specificactivity under that condition was 7.5 Unit/ ml and 19.7 Unit/ mg, respectively.
pH of the medium of maximuminulinase activity 8.3 Unit/ ml and specific activity 20.6 Unit/ mg were obtained at pH 7.
Increase in the enzymeactivity was observed as the shaking speed increase from 50-250 rpm, with the maximum inulinase activity at 200rpm.
It was revealed that the enzyme had the efficiency to hydrolyze 85% of 5% inulin solution when treated at 40 ºC for 80 min.
American Psychological Association (APA)
Awdah, Jasim Muhammad. 2020. Improve of indulines enzyme productivity from recombinant Escherichia coli BL21 (DE3). Mesopotamia Environment Journal،Vol. 5, no. 4, pp.51-57.
https://search.emarefa.net/detail/BIM-978659
Modern Language Association (MLA)
Awdah, Jasim Muhammad. Improve of indulines enzyme productivity from recombinant Escherichia coli BL21 (DE3). Mesopotamia Environment Journal Vol. 5, no. 4 (2020), pp.51-57.
https://search.emarefa.net/detail/BIM-978659
American Medical Association (AMA)
Awdah, Jasim Muhammad. Improve of indulines enzyme productivity from recombinant Escherichia coli BL21 (DE3). Mesopotamia Environment Journal. 2020. Vol. 5, no. 4, pp.51-57.
https://search.emarefa.net/detail/BIM-978659
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 56-57
Record ID
BIM-978659