Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal)‎ against Certain Wound-Infective Gram-Negative Bacteria

Joint Authors

Patel, Pooja
Joshi, Chinmayi
Kothari, Vijay

Source

Advances in Pharmacological and Pharmaceutical Sciences

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-17, 17 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-01-27

Country of Publication

Egypt

No. of Pages

17

Main Subjects

Pharmacology

Abstract EN

This study investigated antipathogenic efficacy of a polyherbal wound-healing formulation Herboheal against three multidrug-resistant strains of gram-negative bacterial pathogens associated with wound infections.

Herboheal was evaluated for its quorum-modulatory potential against three different human-pathogenic bacteria, first in vitro through the broth dilution assay and then in vivo in the model host Caenorhabditis elegans.

Herboheal at ≥0.1% v/v was able to inhibit (19–55%) in vitro production of quorum sensing-regulated pigments in all these bacteria and seemed to interfere with bacterial quorum sensing by acting as a signal-response inhibitor.

This formulation could compromise haemolytic activity of all three bacteria by ∼18–69% and induced their catalase activity by ∼8–21%.

Herboheal inhibited P.

aeruginosa biofilm formation up to 40%, reduced surface hydrophobicity of P.

aeruginosa cells by ∼9%, and also made them (25%) more susceptible to lysis by human serum.

Antibiotic susceptibility of all three bacteria was modulated owing to pretreatment with Herboheal.

Exposure of these test pathogens to Herboheal (≥0.025% v/v) effectively reduced their virulence towards the nematode Caenorhabditis elegans.

Repeated subculturing of P.

aeruginosa on the Herboheal-supplemented growth medium did not induce resistance to Herboheal in this mischievous pathogen, and this polyherbal extract was also found to exert a post-extract effect on P.

aeruginosa, wherein virulence of the Herboheal-unexposed daughter cultures, of the Herboheal-exposed parent culture, was also found to be attenuated.

Overall, this study indicates Herboheal formulation to be an effective antipathogenic preparation and validates its indicated traditional therapeutic use as a wound-care formulation.

American Psychological Association (APA)

Patel, Pooja& Joshi, Chinmayi& Kothari, Vijay. 2019. Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal) against Certain Wound-Infective Gram-Negative Bacteria. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-986289

Modern Language Association (MLA)

Patel, Pooja…[et al.]. Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal) against Certain Wound-Infective Gram-Negative Bacteria. Advances in Pharmacological and Pharmaceutical Sciences No. 2019 (2019), pp.1-17.
https://search.emarefa.net/detail/BIM-986289

American Medical Association (AMA)

Patel, Pooja& Joshi, Chinmayi& Kothari, Vijay. Antipathogenic Potential of a Polyherbal Wound-Care Formulation (Herboheal) against Certain Wound-Infective Gram-Negative Bacteria. Advances in Pharmacological and Pharmaceutical Sciences. 2019. Vol. 2019, no. 2019, pp.1-17.
https://search.emarefa.net/detail/BIM-986289

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-986289