Antimutagenic and Synergistic Cytotoxic Effect of Cisplatin and Honey Bee Venom on 4T1 Invasive Mammary Carcinoma Cell Line

Joint Authors

Shiassi Arani, Faranak
Karimzadeh, Latifeh
Ghafoori, Seyed Mohammad
Nabiuni, Mohammad

Source

Advances in Pharmacological and Pharmaceutical Sciences

Issue

Vol. 2019, Issue 2019 (31 Dec. 2019), pp.1-8, 8 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2019-01-29

Country of Publication

Egypt

No. of Pages

8

Main Subjects

Pharmacology

Abstract EN

Honey bee venom (HBV) has various biological activities such as the inhibitory effect on several types of cancer.

Cisplatin is an old and potent drug to treat most of the cancers.

Our aim in the present study was to determine antimutagenic and cytotoxic effects of HBV on mammary carcinoma, exclusively and in combination with cisplatin.

Methods.

In this study, 4T1 cell line was cultured in RPMI-1640 with 10% fetal bovine serum (FBS), at 37°C in humidified CO2 incubator.

The cell viabilities were examined by the MTT assay.

Also, HBV was screened for its antimutagenic activity via the Ames test.

The results were assessed by SPSS software version 19 and one-way ANOVA method considering p<0.05 level of significance.

Results.

The results showed that 6 mg/ml of HBV, 20 μg/ml of cisplatin, and 6 mg/ml HBV with 10 μg/ml cisplatin could induce approximately 50% of 4T1 cell death.

The concentration 7 mg/ml of HBV with of 62.76% inhibitory rate showed the highest antimutagenic activity in comparison with other treatment groups.

Conclusions.

The MTT assay demonstrated that HBV and cisplatin could cause cell death in a dose-dependent manner.

The cytotoxic effect of cisplatin also promoted by HBV.

Ames test outcomes indicated that HBV could act as a significant mutagenic agent.

The antimutagenic activity of HBV was increased considerably in the presence of S9 mix.

Therefore, our findings have revealed that HBV can enhance the cytotoxic effect of cisplatin drug and its cancer-preventing effects.

American Psychological Association (APA)

Shiassi Arani, Faranak& Karimzadeh, Latifeh& Ghafoori, Seyed Mohammad& Nabiuni, Mohammad. 2019. Antimutagenic and Synergistic Cytotoxic Effect of Cisplatin and Honey Bee Venom on 4T1 Invasive Mammary Carcinoma Cell Line. Advances in Pharmacological and Pharmaceutical Sciences،Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-986302

Modern Language Association (MLA)

Shiassi Arani, Faranak…[et al.]. Antimutagenic and Synergistic Cytotoxic Effect of Cisplatin and Honey Bee Venom on 4T1 Invasive Mammary Carcinoma Cell Line. Advances in Pharmacological and Pharmaceutical Sciences No. 2019 (2019), pp.1-8.
https://search.emarefa.net/detail/BIM-986302

American Medical Association (AMA)

Shiassi Arani, Faranak& Karimzadeh, Latifeh& Ghafoori, Seyed Mohammad& Nabiuni, Mohammad. Antimutagenic and Synergistic Cytotoxic Effect of Cisplatin and Honey Bee Venom on 4T1 Invasive Mammary Carcinoma Cell Line. Advances in Pharmacological and Pharmaceutical Sciences. 2019. Vol. 2019, no. 2019, pp.1-8.
https://search.emarefa.net/detail/BIM-986302

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-986302