Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry
Joint Authors
Kabbage, Maria
Hoebeke, Johan
Remadi, Sami
Chouchane, Lotfi
Trimeche, Mounir
Guillier, Christelle Lemaitre
Hamrita, Bechr
Chahed, Karim
Source
Issue
Vol. 2008, Issue 2008 (31 Dec. 2008), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2008-02-17
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Improvement of breast-cancer detection through the identification of potential cancer biomarkers is considered as a promising strategy for effective assessment of the disease.
The current study has used nonequilibrium pH gradient electrophoresis with subsequent analysis by mass spectrometry to identify protein alterations in invasive ductal carcinomas of the breast from Tunisian women.
We have identified multiple protein alterations in tumor tissues that were picked, processed, and unambiguously assigned identities by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF).
The proteins identified span a wide range of functions and are believed to have potential clinical applications as cancer biomarkers.
They include glycolytic enzymes, molecular chaperones, cytoskeletal-related proteins, antioxydant enzymes, and immunologic related proteins.
Among these proteins, enolase 1, phosphoglycerate kinase 1, deoxyhemoglobin, Mn-superoxyde dismutase, α-B-crystallin, HSP27, Raf kinase inhibitor protein, heterogeneous nuclear ribonucleoprotein A2/B1, cofilin 1, and peptidylprolyl isomerase A were overexpressed in tumors compared with normal tissues.
In contrast, the IGHG1 protein, the complement C3 component C3c, which are two newly identified protein markers, were downregulated in IDCA tissues.
American Psychological Association (APA)
Kabbage, Maria& Chahed, Karim& Hamrita, Bechr& Guillier, Christelle Lemaitre& Trimeche, Mounir& Remadi, Sami…[et al.]. 2008. Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry. BioMed Research International،Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-987788
Modern Language Association (MLA)
Kabbage, Maria…[et al.]. Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry. BioMed Research International No. 2008 (2008), pp.1-10.
https://search.emarefa.net/detail/BIM-987788
American Medical Association (AMA)
Kabbage, Maria& Chahed, Karim& Hamrita, Bechr& Guillier, Christelle Lemaitre& Trimeche, Mounir& Remadi, Sami…[et al.]. Protein Alterations in Infiltrating Ductal Carcinomas of the Breast as Detected by Nonequilibrium pH Gradient Electrophoresis and Mass Spectrometry. BioMed Research International. 2008. Vol. 2008, no. 2008, pp.1-10.
https://search.emarefa.net/detail/BIM-987788
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-987788