Development of a Novel Bioinformatics Tool for In Silico Validation of Protein Interactions
Joint Authors
Comincini, Sergio
Azzalin, Alberto
Bellazzi, Riccardo
Barbarini, Nicola
Simonelli, Luca
Source
Issue
Vol. 2010, Issue 2010 (31 Dec. 2009), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-05-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Protein interactions are crucial in most biological processes.
Several in silico methods have been recently developed to predict them.
This paper describes a bioinformatics method that combines sequence similarity and structural information to support experimental studies on protein interactions.
Given a target protein, the approach selects the most likely interactors among the candidates revealed by experimental techniques, but not yet in vivo validated.
The sequence and the structural information of the in vivo confirmed proteins and complexes are exploited to evaluate the candidate interactors.
Finally, a score is calculated to suggest the most likely interactors of the target protein.
As an example, we searched for GRB2 interactors.
We ranked a set of 46 candidate interactors by the presented method.
These candidates were then reduced to 21, through a score threshold chosen by means of a cross-validation strategy.
Among them, the isoform 1 of MAPK14 was in silico confirmed as a GRB2 interactor.
Finally, given a set of already confirmed interactors of GRB2, the accuracy and the precision of the approach were 75% and 86%, respectively.
In conclusion, the proposed method can be conveniently exploited to select the proteins to be experimentally investigated within a set of potential interactors.
American Psychological Association (APA)
Barbarini, Nicola& Simonelli, Luca& Azzalin, Alberto& Comincini, Sergio& Bellazzi, Riccardo. 2010. Development of a Novel Bioinformatics Tool for In Silico Validation of Protein Interactions. BioMed Research International،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989057
Modern Language Association (MLA)
Barbarini, Nicola…[et al.]. Development of a Novel Bioinformatics Tool for In Silico Validation of Protein Interactions. BioMed Research International No. 2010 (Dec. 2010), pp.1-9.
https://search.emarefa.net/detail/BIM-989057
American Medical Association (AMA)
Barbarini, Nicola& Simonelli, Luca& Azzalin, Alberto& Comincini, Sergio& Bellazzi, Riccardo. Development of a Novel Bioinformatics Tool for In Silico Validation of Protein Interactions. BioMed Research International. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-989057
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989057