Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning
Joint Authors
Hassan, Moustapha
Rozell, Bjorn
Hassan, Zuzana
al-Hashmi, Suleiman
Concha, Hernan
Lundmark, Carin
Grönvik, Kjell-Olov
Abedi-Valugerdi, Manuchehr
Sadeghi, Behnam
Source
Journal of Immunology Research
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-11-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
In the present paper, we have investigated early pathophysiological events in graft-versus-host disease (GVHD), a major complication to hematopoietic stem cell transplantation (HSCT).
BLLB/c female mice conditioned with busulfan/cyclophosphamide (Bu-Cy) were transplanted with allogeneic male C57BL/6.
Control group consisted of syngeneic transplanted Balb/c mice.
In allogeneic settings, significant expansion and maturation of donor dendritic cells (DCs) were observed at day +3, while donor T-cells CD8+ were increased at day +5 (230%) compared to syngeneic HSCT.
Highest levels of inflammatory cytokines IL-2, IFN-gamma, and TNF-alfa at day +5 matched T-cell activation.
Concomitantly naïve T-cells gain effecr-memory phenotype and migrated from spleen to peripheral lymphoid organs.
Thus, in the very early phase of GHVD following Bu-Cy conditioning donor, DCs play an important role in the activation of donor T cells.
Subsequently, donor naïve T-cells gain effector-memory phenotype and initiate GVHD.
American Psychological Association (APA)
Sadeghi, Behnam& al-Hashmi, Suleiman& Hassan, Zuzana& Rozell, Bjorn& Concha, Hernan& Lundmark, Carin…[et al.]. 2010. Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning. Journal of Immunology Research،Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989286
Modern Language Association (MLA)
Sadeghi, Behnam…[et al.]. Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning. Journal of Immunology Research No. 2010 (2010), pp.1-13.
https://search.emarefa.net/detail/BIM-989286
American Medical Association (AMA)
Sadeghi, Behnam& al-Hashmi, Suleiman& Hassan, Zuzana& Rozell, Bjorn& Concha, Hernan& Lundmark, Carin…[et al.]. Expansion and Activation Kinetics of Immune Cells during Early Phase of GVHD in Mouse Model Based on Chemotherapy Conditioning. Journal of Immunology Research. 2010. Vol. 2010, no. 2010, pp.1-13.
https://search.emarefa.net/detail/BIM-989286
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-989286