Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells
Joint Authors
Resnik, Nataša
Mrak-Poljšak, Katjuša
Veranič, Peter
Lakota, Katja
Sodin-Semrl, Snezna
Source
Issue
Vol. 2011, Issue 2011 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-10-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Serum amyloid A (SAA) acts as a major acute phase protein and represents a sensitive and accurate marker of inflammation.
Besides its hepatic origin, as the main source of serum SAA, this protein is also produced extrahepatically.
The mRNA levels of SAA become significantly elevated following proinflammatory stimuli, as well as, are induced through their own positive feedback in human primary coronary artery endothelial cells.
However, the intracellular functions of SAA are so far unknown.
Colocalization of SAA with cytoskeletal filaments has previously been proposed, so we analyzed the colocalization of SAA with all three cytoskeletal elements: actin filaments, vimentin filaments, and microtubules.
Immunofluorescent double-labeling analyses confirmed by PLA method revealed a strict colocalization of SAA with microtubules and a very infrequent attachment to vimentin while the distribution of actin filaments appeared clearly separated from SAA staining.
Also, no significant colocalization was found between SAA and endomembranes labeled with the fluorescent lipid stain DiO6.
However, SAA appears to be located also unbound in the cytosol, as well as inside the nucleus and within nanotubes extending from the cells or bridging neighboring cells.
These different locations of SAA in endothelial cells strongly indicate multiple potential functions of this protein.
American Psychological Association (APA)
Lakota, Katja& Resnik, Nataša& Mrak-Poljšak, Katjuša& Sodin-Semrl, Snezna& Veranič, Peter. 2011. Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990181
Modern Language Association (MLA)
Lakota, Katja…[et al.]. Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990181
American Medical Association (AMA)
Lakota, Katja& Resnik, Nataša& Mrak-Poljšak, Katjuša& Sodin-Semrl, Snezna& Veranič, Peter. Colocalization of Serum Amyloid A with Microtubules in Human Coronary Artery Endothelial Cells. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-990181