7α-Hydroxy-β-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular BaxBcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK12 Activation
Joint Authors
Tasyriq, Mohammad
Najmuldeen, Ibrahim A.
In, Lionel L. A.
Mohamad, Khalit
Nagoor, Noor Hasima
Awang, Khalijah
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-11
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
In continuation of our interest towards the elucidation of apoptotic pathways of cytotoxic phytocompounds, we have embarked upon a study on the anticancer effects of 7α-hydroxy-β-sitosterol (CT1), a rare natural phytosterol oxide isolated from Chisocheton tomentosus.
CT1 was found to be cytotoxic on three different human tumor cell lines with minimal effects on normal cell controls, where cell viability levels were maintained ≥80% upon treatment.
Our results showed that cell death in MCF-7 breast tumor cells was achieved through the induction of apoptosis via downregulation of the ERK1/2 signaling pathway.
CT1 was also found to increase proapoptotic Bax protein levels, while decreasing anti-apoptotic Bcl-2 protein levels, suggesting the involvement of the intrinsic pathway.
Reduced levels of initiator procaspase-9 and executioner procaspase-3 were also observed following CT1 exposure, confirming the involvement of cytochrome c-mediated apoptosis via the mitochondrial pathway.
These results demonstrated the cytotoxic and apoptotic ability of 7α-hydroxy-β-sitosterol and suggest its potential anti-cancer use particularly on breast adenocarcinoma cells.
American Psychological Association (APA)
Tasyriq, Mohammad& Najmuldeen, Ibrahim A.& In, Lionel L. A.& Mohamad, Khalit& Awang, Khalijah& Nagoor, Noor Hasima. 2012. 7α-Hydroxy-β-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular BaxBcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK12 Activation. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-992625
Modern Language Association (MLA)
Tasyriq, Mohammad…[et al.]. 7α-Hydroxy-β-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular BaxBcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK12 Activation. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-992625
American Medical Association (AMA)
Tasyriq, Mohammad& Najmuldeen, Ibrahim A.& In, Lionel L. A.& Mohamad, Khalit& Awang, Khalijah& Nagoor, Noor Hasima. 7α-Hydroxy-β-Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular BaxBcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK12 Activation. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-992625
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992625