Detection for MECA gene in Egyptian clinical Staphylococcus aureus samples and study their genotoxic activity
Other Title(s)
الكشف عن جين ال MecA في عزلات البكتريا العنقودية المصرية و دراسة السمية الوراثية الخاصة بها
Joint Authors
Ibrahim, Samir Abd al-Aziz
al-Atiq, Sawsan Yusuf
Abd al-Raziq, Ashraf Bakri
Hasan, Marwah
Source
Arab Universities Journal of Agricultural Sciences
Publisher
Ain Shams University Faculty of Agriculture
Publication Date
2019-03-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Topics
- Activities
- Tests
- Mutation
- Bone marrow
- Damages
- Nucleic acids
- Control systems
- University hospitals
- DNA
- Infection control
- Genetic aspects
- Polymerase chain reaction
- Gel electrophoresis
- Staphylococcus
English Abstract
Staphylococcus aureus isolates were isolated from fifty clinical samples collected from Ain Shams University Hospitals (March - July 2016) and identified using biochemical and microbiological tests.
PCR was performed using specific primers to determine the isolates of Methicillin - Resistant Staphylococcus aureus (MRSA) based on the presence of mecA gene.
Thirty-four isolates from total fifty isolates (68% ) were identified as MRSA isolates.
To study the genotoxicity for this isolates, forty-five mice were injected with these MRSA isolates and comet and micronuclei assays were performed on mice liver tissues and bone marrow respectively.
These assays revealed 24% - 22% DNA damage as an indicator for chromosomal breakage by comet and micronucleus assays respectively which indicate that infection with these isolates leads to mutations.
Studying these isolate furthermore will give an insight on how critical maintaining high standard hygiene in Egyptian hospitals and attention to infection control system can prevent occur of outbreaks
Data Type
Conference Papers
Record ID
BIM-999839
American Psychological Association (APA)
Hasan, Marwah& Ibrahim, Samir Abd al-Aziz& Abd al-Raziq, Ashraf Bakri& al-Atiq, Sawsan Yusuf. 2019-03-31. Detection for MECA gene in Egyptian clinical Staphylococcus aureus samples and study their genotoxic activity. . Vol. 27, no. 1 (s) (2019), pp.529-537.Cairo Egypt : Ain Shams University Faculty of Agriculture.
https://search.emarefa.net/detail/BIM-999839
Modern Language Association (MLA)
Hasan, Marwah…[et al.]. Detection for MECA gene in Egyptian clinical Staphylococcus aureus samples and study their genotoxic activity. . Cairo Egypt : Ain Shams University Faculty of Agriculture. 2019-03-31.
https://search.emarefa.net/detail/BIM-999839
American Medical Association (AMA)
Hasan, Marwah& Ibrahim, Samir Abd al-Aziz& Abd al-Raziq, Ashraf Bakri& al-Atiq, Sawsan Yusuf. Detection for MECA gene in Egyptian clinical Staphylococcus aureus samples and study their genotoxic activity. .
https://search.emarefa.net/detail/BIM-999839