In Vitro Antioxidant and Cancer Inhibitory Activity of a Colored Avocado Seed Extract

الملخص EN

Avocado (Persea americana) seeds have been used traditionally for a number of health-related indications.

Because of its high polyphenol content, we investigated the potential antioxidant and anticancer effects of a colored avocado seed extract (CASE).

CASE exhibited an oxygen radical acceptance capacity value of 2012 ± 300 trolox equivalents/mg.

CASE reduced lipid hydroperoxide formation in an oil-in-water emulsion (33% reduction at 500 μg/mL).

CASE dose-dependently reduced the viability of human breast (MCF7), lung (H1299), colon (HT29), and prostate (LNCaP) cancer cells in vitro.

The half maximal inhibitory concentrations ranged from 19 to 132 μg/mL after treatment for 48 h.

CASE treatment downregulated the expression of cyclin D1 and E2 in LNCaP cells.

This was associated with cell G0/G1 phase cycle arrest.

CASE also dose-dependently induced apoptosis in LNCaP cells.

CASE reduced nuclear translocation of nuclear factor κB, a prosurvival signal.

Further studies are needed to examine these effects in in vivo models.