Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones
المؤلفون المشاركون
Barros-Velázquez, Jorge
Calo-Mata, Pilar
Quintela-Baluja, Marcos
Fernández-No, Inmaculada
Böhme, Karola
Benmechernene, Zineb
Kihal, Mebrouk
المصدر
العدد
المجلد 2014، العدد 2014 (31 ديسمبر/كانون الأول 2014)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2014-04-07
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Information on the microbiology of camel milk is very limited.
In this work, the genetic characterization and proteomic identification of 13 putative producing bacteriocin Leuconostoc strains exhibiting antilisterial activity and isolated from camel milk were performed.
DNA sequencing of the 13 selected strains revealed high homology among the 16S rRNA genes for all strains.
In addition, 99% homology with Leuconostoc mesenteroides was observed when these sequences were analysed by the BLAST tool against other sequences from reference strains deposited in the Genbank.
Furthermore, the isolates were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) which allowed for the identification of 2 mass peaks 6242 m/z and 5118 m/z that resulted to be specific to the species L.
mesenteroides.
Remarkably, the phyloproteomic tree provided more intraspecific information of L.
mesenteroides than phylogenetic analysis.
Accordingly, phyloproteomic analysis grouped L.
mesenteroides strains into different subbranches, while all L.
mesenteroides isolates were grouped in the same branch according to phylogenetic analysis.
This study represents, to our knowledge, the first report on the use of MALDI-TOF MS on the identification of LAB isolated from camel milk.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Benmechernene, Zineb& Fernández-No, Inmaculada& Quintela-Baluja, Marcos& Böhme, Karola& Kihal, Mebrouk& Calo-Mata, Pilar…[et al.]. 2014. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503491
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Benmechernene, Zineb…[et al.]. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-503491
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Benmechernene, Zineb& Fernández-No, Inmaculada& Quintela-Baluja, Marcos& Böhme, Karola& Kihal, Mebrouk& Calo-Mata, Pilar…[et al.]. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503491
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-503491
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر