Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones
Joint Authors
Barros-Velázquez, Jorge
Calo-Mata, Pilar
Quintela-Baluja, Marcos
Fernández-No, Inmaculada
Böhme, Karola
Benmechernene, Zineb
Kihal, Mebrouk
Source
Issue
Vol. 2014, Issue 2014 (31 Dec. 2014), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2014-04-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Information on the microbiology of camel milk is very limited.
In this work, the genetic characterization and proteomic identification of 13 putative producing bacteriocin Leuconostoc strains exhibiting antilisterial activity and isolated from camel milk were performed.
DNA sequencing of the 13 selected strains revealed high homology among the 16S rRNA genes for all strains.
In addition, 99% homology with Leuconostoc mesenteroides was observed when these sequences were analysed by the BLAST tool against other sequences from reference strains deposited in the Genbank.
Furthermore, the isolates were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDITOF MS) which allowed for the identification of 2 mass peaks 6242 m/z and 5118 m/z that resulted to be specific to the species L.
mesenteroides.
Remarkably, the phyloproteomic tree provided more intraspecific information of L.
mesenteroides than phylogenetic analysis.
Accordingly, phyloproteomic analysis grouped L.
mesenteroides strains into different subbranches, while all L.
mesenteroides isolates were grouped in the same branch according to phylogenetic analysis.
This study represents, to our knowledge, the first report on the use of MALDI-TOF MS on the identification of LAB isolated from camel milk.
American Psychological Association (APA)
Benmechernene, Zineb& Fernández-No, Inmaculada& Quintela-Baluja, Marcos& Böhme, Karola& Kihal, Mebrouk& Calo-Mata, Pilar…[et al.]. 2014. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International،Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503491
Modern Language Association (MLA)
Benmechernene, Zineb…[et al.]. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International No. 2014 (2014), pp.1-10.
https://search.emarefa.net/detail/BIM-503491
American Medical Association (AMA)
Benmechernene, Zineb& Fernández-No, Inmaculada& Quintela-Baluja, Marcos& Böhme, Karola& Kihal, Mebrouk& Calo-Mata, Pilar…[et al.]. Genomic and Proteomic Characterization of Bacteriocin-Producing Leuconostoc mesenteroides Strains Isolated from Raw Camel Milk in Two Southwest Algerian Arid Zones. BioMed Research International. 2014. Vol. 2014, no. 2014, pp.1-10.
https://search.emarefa.net/detail/BIM-503491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-503491