Antibacterial and antioxidant activities of methanolic extracts of astragalus sieberi, ballota undulata, varthemia iphionoides, linum mucronatum and phlomis armeniaca selected from southern jordan
Other Title(s)
الأنشطة المضادة للبكتيريا و المضادة للأكسدة في مستخلصات الميثانول لنباتات خنصر العروس, القرطم, الشتيله, الكتان الأصفر و الاذانة الصفراء المنتقاة من جنوب الأردن
Dissertant
Thesis advisor
al-Tarawinah, Khalid Ahmad Jafar
Comitee Members
al-Zaraini, Wail Ali Hasan
al-Shakanbah, Jumah Muti
al-Shahatit, Sulhi Faysal
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2013
English Abstract
In the present study, methanol extracts of Astragalus sieberi, Ballota undulata, Varthemia iphionoides, Linum mucronatum and Phlomis armeniaca were examined for its antioxidant and antibacterial activities for five bacterial strains (S.
aureus, E.
coli, K.
pneumoniae, B.
subtilis, and M.
luteus).
The antibacterial activity for K.
pneumoniae (Gram negative bacteria) and S.
aureus B.
subtilis and M.
luteus (Gram positive bacteria) was reported with the extract of A.
sieberi, V.
iphionoides, and P.
armeniaca, while A.
sieberi showed the highest antibacterial activity for K.
pneumoniae, S.
aureus, B.
subtilis and M.
luteus.
The minimum inhibitory concentrations (MICs) were 250, 500, 250 and 250 µg / ml, respectively, compared to A.
sieberi, where MeOH extracts of P.
armeniaca and V.
iphionoides showed a lower antibacterial activity.
The extracts from B.
Undulata and L.
mucronatum did not show any antibacterial activity against these bacterial strains.
The total plant phenol content in dry extract, of V.
iphionoides, and P.
armeniaca were found 205 and 188 mg GAE / g respectively, while A.
sieberi, B.
undulate and L.
mucronatum were 66, 87 and 74 mg GAE / g, respectively.
The DPPH method was used to measure the radical scavenging activity of plant extracts, whereas, the FRAP was used to measure the ferric reducing antioxidant power.
The highest free radical scavenging activities (reflected by the smallest IC50 values) were exerted by P.
armeniaca and V.
iphionoides extract (1.07 and 1.11 mg Trolox / ml, respectively).
The lowest radical scavenging activity was exhibited by L.
mucronatum, A.
sieberi and B.
undulata (IC50 : 9.84, 9.49 and 3.2 mg Trolox / ml, respectively).
Same trends were appeared when the above plants tested in FRAP for antioxidant activity.
High positive correlation was appeared between the total phenolic content and the antioxidant activity calculated TEACDPPH and TEACFRAP methods.
This correlation was expressed by the correlation coefficient (R), which was 0.987 and 0.93 for the TEACDPPH and TEACFRAP, respectively.
Both methods, showed a high correlation with TPCs, indicating that both methods are efficient for antioxidant measurements.
Main Subjects
Topics
No. of Pages
65
Table of Contents
Table of contents.
Abstract.
Chapter One : theoretical background.
Chapter Two : literature review.
Chapter Three : design and methodology.
Chapter Four : results, discussion and recommendation.
References.
American Psychological Association (APA)
al-Qaysi, Yasin Taha. (2013). Antibacterial and antioxidant activities of methanolic extracts of astragalus sieberi, ballota undulata, varthemia iphionoides, linum mucronatum and phlomis armeniaca selected from southern jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-322719
Modern Language Association (MLA)
al-Qaysi, Yasin Taha. Antibacterial and antioxidant activities of methanolic extracts of astragalus sieberi, ballota undulata, varthemia iphionoides, linum mucronatum and phlomis armeniaca selected from southern jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2013).
https://search.emarefa.net/detail/BIM-322719
American Medical Association (AMA)
al-Qaysi, Yasin Taha. (2013). Antibacterial and antioxidant activities of methanolic extracts of astragalus sieberi, ballota undulata, varthemia iphionoides, linum mucronatum and phlomis armeniaca selected from southern jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-322719
Language
English
Data Type
Arab Theses
Record ID
BIM-322719
