Evaluation of antibacterial and antioxidant properties of methanolic extracts of sarcopoterium spinosum, paronychia argentea, inula viscosa, and achillea fragrantissima selected from Wadi al-Karak–Jordan
Other Title(s)
تقييم أثر المضادات البكتيرية و مضادات الأكسدة للمستخلصات الميثانولية لنباتات البلان، رجل الحمام، الطيون و القيصوم و التي تم اختيارها من منطقة وادي الكرك-الأردن
Dissertant
Thesis advisor
al-Tarawinah, Khalid Ahmad Jafar
Comitee Members
al-Shakanbah, Jumah Muti
al-Shahatit, Sulhi Faysal
Atruz, Umar Muhammad Falih
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2013
English Abstract
Antibacterial and antioxidant properties of methanolic extracts derived from aerial parts of four Jordanian medicinal plants (Sarcopoterium spinosum, Paronychia argentea, Inula viscosa, and Achillea fragrantissima) were investigated in this study.
Serial dilution and disc diffusion methods were performed to measure the antibacterial activity of these extracts against different bacterial strains (S.
aureus, E.
coli, K.
pneumoniae, B.
subtilis and M.
luteus).
The total phenolic content was assessed by Folin-Ciocalteau method.
The amount of the extract required to scavenge 50% of DPPH radical (IC50) was also measured.
The antioxidant capacities was assessed by both DPPH and FRAP methods.
The possible relationship between the antioxidant capacity and the total phenolic content of these extracts was also determined.
Variations were observed among the examined plants in antibacterial and antioxidant activities as well as in their phenol contents.
The results of antibacterial investigation showed that I.
viscosa exhibited the highest antibacterial activity against all tested bacterial strains as compared to S.
spinosum, A.
fragrantissima and P.
argentea.
S.
spinosum contained the highest amount of phenolic compounds (320 mg GA g-1 plant extract) followed by I.
viscosa, A.
fragrantissima, and P.
argentea (258, 87, and 83 mg GA g-1 plant extract, respectively).
According to the IC50 values of DPPH assay, the highest free radical scavenging potential was found in S.
spinosum (0.3 mg mL-1), followed by I.
viscosa, A.
fragrantissima, and P.
argentea (0.58, 3.06, and 3.42 mg mL-1, respectively).
The trend for ferric ions reducing activities of the four plant extracts did not vary markedly from their DPPH free radical scavenging activities.
In conclusion, these plants are not only interesting sources for antimicrobial agents but also have a considerable amount of antioxidants.
In addition, these findings revealed that the antioxidant capacity of these plant extracts could be attributed to their total phenolic content.
Main Subjects
Topics
No. of Pages
57
Table of Contents
Table of contents.
Abstract.
Chapter One : theoretical background.
Chapter Two : literature review.
Chapter Three : design and methodology.
Chapter Four : results, discussion and recommendation.
References.
American Psychological Association (APA)
al-Farayah, Ibrahim Ismail. (2013). Evaluation of antibacterial and antioxidant properties of methanolic extracts of sarcopoterium spinosum, paronychia argentea, inula viscosa, and achillea fragrantissima selected from Wadi al-Karak–Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-322725
Modern Language Association (MLA)
al-Farayah, Ibrahim Ismail. Evaluation of antibacterial and antioxidant properties of methanolic extracts of sarcopoterium spinosum, paronychia argentea, inula viscosa, and achillea fragrantissima selected from Wadi al-Karak–Jordan. (Master's theses Theses and Dissertations Master). Mutah University. (2013).
https://search.emarefa.net/detail/BIM-322725
American Medical Association (AMA)
al-Farayah, Ibrahim Ismail. (2013). Evaluation of antibacterial and antioxidant properties of methanolic extracts of sarcopoterium spinosum, paronychia argentea, inula viscosa, and achillea fragrantissima selected from Wadi al-Karak–Jordan. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-322725
Language
English
Data Type
Arab Theses
Record ID
BIM-322725
