Antioxidative,antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract
Joint Authors
Rahman, Attar
Bin Umran, Talhat
Islam, Shahid
Source
Saudi Journal of Biological Sciences
Issue
Vol. 20, Issue 3 (31 Jul. 2013), pp.213-225, 13 p.
Publisher
Publication Date
2013-07-31
Country of Publication
Saudi Arabia
No. of Pages
13
Main Subjects
Topics
Abstract EN
This study investigated the phytochemical, antioxidative, antimicrobial and cytotoxic effects of Leea indica leaf ethanol extract.
Phytochemical values namely total phenolic and flavonoid contents, total antioxidant capacity, DPPH radical scavenging effect, FeCl3 reducing power, DMSO superoxide scavenging effect and Iron chelating effects were studied by established methods.
Antibacterial, antifungal and cytotoxic effects were screened by disk diffusion technique, food poison technique and brine shrimp bioassay, respectively.
Results showed the total phenolic content 24.00 ±0.81 g GAE / 100 g, total flavonoid content 194.68± 2.43 g quercetin / 100 g and total antioxidant capacity 106.61 ± 1.84 g AA / 100 g dry extract.
Significant (P < 0.05) IC50 values compared to respective standards were recorded in DPPH radical scavenging (139.83 ± 1.40 lg / ml), FeCl3 reduction (16.48 ± 0.64 lg / ml), DMSO superoxide scavenging (676.08 ± 5.80 lg / ml) and Iron chelating (519.33 ± 16.96 lg / ml) methods.
In antibacterial screening, the extract showed significant (P < 0.05) zone of inhibitions compared to positive controls Ampicillin and Tetracycline against Gram positive Bacillus subtilis, Bacillus cereus, Bacillus megaterium, and Staphylococcus aureus and Gram negative Salmonella typhi, Salmonella paratyphi, Pseudomonas aeroginosa, Shigella dysenteriae, Vibrio cholerae, and Escherichia coli.
Significant minimum inhibitory concentrations compared to tetracycline were obtained against the above organisms.
In antifungal assay, the extract inhibited the growth of Aspergillus flavus, Candida albicans and Fusarium equisetii by 38.09 ± 0.59, 22.58 ±2.22, and 22.58 ± 2.22 %, respectively.
The extract showed a significant LC50 value compared to vincristine sulfate in cytotoxic assay.
The results evidenced the potential.
American Psychological Association (APA)
Rahman, Attar& Bin Umran, Talhat& Islam, Shahid. 2013. Antioxidative,antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract. Saudi Journal of Biological Sciences،Vol. 20, no. 3, pp.213-225.
https://search.emarefa.net/detail/BIM-358248
Modern Language Association (MLA)
Rahman, Attar…[et al.]. Antioxidative,antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract. Saudi Journal of Biological Sciences Vol. 20, no. 3 (Jul. 2013), pp.213-225.
https://search.emarefa.net/detail/BIM-358248
American Medical Association (AMA)
Rahman, Attar& Bin Umran, Talhat& Islam, Shahid. Antioxidative,antimicrobial and cytotoxic effects of the phenolics of leea indica leaf extract. Saudi Journal of Biological Sciences. 2013. Vol. 20, no. 3, pp.213-225.
https://search.emarefa.net/detail/BIM-358248
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 223-225
Record ID
BIM-358248