Detection of non typhoidal Salmonella isolated from food products and clinical cases by conventional bacteriological methods in Sohag Governorates

Abstract EN

The aim of the present work is to compare different conventional methods for identification of non-typhoidal Salmonella species and determine the virulence of the salmonella serovars obtained from animal sources.

Non typhoidal Salmonella (NTS) are important food-borne pathogens.

Infection with NTS may not lead to fatal disease, but it may remain localized in the gastrointestinal tract resulting in gastroenteritis or my take a septicemic from that can affect several organ systems causing gastroenteritis, bacteremia and subsequent focal infection.

A total of 390 examined samples were collected, these included : 100 milk, 100 eggs and 90 fresh slaughtered chickens from farms, slaughterhouses, markets as well as 100 feces samples from acute enteric infected cattle.

They were subjected to conventional methods for bacteriological, biochemical and serological examination.

The culture methods detect 41 positive Salmonella species, while API 20 E could detect only 39 of these positive cultures.

Using slide agglutination test, serotyping of the isolated strains according to somatic (O) and flagellar (H) antigens detected presence of 16 S.

enteritidis (39 %) ; 14 S.

typhimurium (34 %) ; 8 S.

infantis (19.5 %) ; 2 S.

kentucky (5 %) ; and 1 S.

tsevie (2.5 %).

It could be concluded that culture, biochemical and serological methods has the ability to detect a wide range of salmonella species.