Improving the production of unsaturated fatty acid esters and fl avonoids from date palm pollen and their effects as anti-breast-cancer and antiviral agents : an in-vitro study
Joint Authors
Ghanimah, Qadri Z.
Ghanim, Hasan Z.
Fadil, Muhammad
Ramadan, Manal M.
Source
Journal of the Arab Society for Medical Research
Issue
Vol. 10, Issue 2 (31 Dec. 2015), pp.47-55, 9 p.
Publisher
Arab Society for Medical Research
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Topics
- Pollen grains
- Virus diseases
- Cancer
- Chemotherapy
- Drugs
- Antiviral agents
- Breast
- Antioxidants
- Gas chromatography
Abstract EN
Background / aim Pollens from different plants contain unsaturated fatty acid esters (USFAEs) and fl avonoids that play a very important role as bioactive compounds.
Therefore, the present study was designed to improve the production of volatile USFAEs and fl avonoids from date palm pollen (DPP) in a culture of Trichoderma koningii and test its activities as an anti-breast-cancer and antiviral agent.
Materials and methods The volatile esters of fermented and nonfermented date palm pollens (FDPPs) were identifi ed using gas chromatographic–mass spectrometric (GC-MS) analysis.
Antioxidant activities were determined using three different methods: the 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assay, the ferric reducing antioxidant power assay, and the 2,2-azinobis(3-ethyl-benzothiazoline-6- sulfonic acid) (ABTS) assay.
Polyphenols (phenolics and fl avonoids) were also determined.
Anti-breast-cancer and antiviral activities were determined using the 3-(4,5-dimethyl-2- thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay.
Results GC–MS analysis showed an improvement in the level of USFAE in FDPP (47.99%) almost double that of the DPP results (24.11%) extract concentration.
Flavonoids content of the FDPP extract (93.4 ± 6.3 mg/ml) was higher than that obtained by the DPP extract (45.4 ± 2.1 mg/ml), which was more than double the value.
Antioxidant activity of the FDPP extract increased 3.16, 3.42, and 2.14 times that of the DPP extract as determined by the ABTS, ferric reducing antioxidant power (FRAP), and DPPH assays, respectively.
The extract of FDPP showed strong anticancer activity against the MCF-7 cell line (IC50: 9.52 μg/ml) compared with the DPP extract (IC50: 96.22 μg/ml).
Also, the FDPP extract had strong antiviral activity (CC50: 16.5 μg/ml) compared with DPP (CC50 : 38.8 μg/ml).
This is the fi rst report in which the FDPP extract is used in biological studies as anti-breast-cancer and antiviral agents.
Conclusion Fermentation of DPP by T.
koningii improves many bioactive volatile USFAE and fl avonoid contents ; these have anti-breast-cancer and antiviral activity.
American Psychological Association (APA)
Ramadan, Manal M.& Ghanim, Hasan Z.& Ghanimah, Qadri Z.& Fadil, Muhammad. 2015. Improving the production of unsaturated fatty acid esters and fl avonoids from date palm pollen and their effects as anti-breast-cancer and antiviral agents : an in-vitro study. Journal of the Arab Society for Medical Research،Vol. 10, no. 2, pp.47-55.
https://search.emarefa.net/detail/BIM-668133
Modern Language Association (MLA)
Ghanimah, Qadri Z.…[et al.]. Improving the production of unsaturated fatty acid esters and fl avonoids from date palm pollen and their effects as anti-breast-cancer and antiviral agents : an in-vitro study. Journal of the Arab Society for Medical Research Vol. 10, no. 2 (Dec. 2015), pp.47-55.
https://search.emarefa.net/detail/BIM-668133
American Medical Association (AMA)
Ramadan, Manal M.& Ghanim, Hasan Z.& Ghanimah, Qadri Z.& Fadil, Muhammad. Improving the production of unsaturated fatty acid esters and fl avonoids from date palm pollen and their effects as anti-breast-cancer and antiviral agents : an in-vitro study. Journal of the Arab Society for Medical Research. 2015. Vol. 10, no. 2, pp.47-55.
https://search.emarefa.net/detail/BIM-668133
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 54-55
Record ID
BIM-668133